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http://dspace.zsmu.edu.ua/handle/123456789/22699
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Название: | The folliclegenesis in the thyroid gland in the postnatal period of ontogenesis under prenatal antigenic influence |
Авторы: | Fedosieieva, O. V. Федосєєва, Ольга Віталіївна |
Ключевые слова: | morphogenesis thyroid gland antigen staphylococcal toxoid experiment |
Дата публикации: | 2021 |
Библиографическое описание: | Fedosieieva O. V. The folliclegenesis in the thyroid gland in the postnatal period of ontogenesis under prenatal antigenic influence / O. V. Fedosieieva // Innovative Solutions In Modern Science. - 2021. - Vol. 3, N 47. - https://doi.org/10.26886/2414-634X.3(47)2021.3. |
Аннотация: | Antigenic influence at critical terms of ontogenesis can cause
significant changes in the child's immune system. It is known that the entry
of antigens into the fetus causes premature release of T-lymphocytes from
the thymus and their migration to various organs. The paper was aimed at
the study of the folliclegenesis of the thyroid gland in postnatal period at
norm and after prenatal influence of staphylococcal toxoid. In the
experimental research as a material were thyroid glands of Wistar rats
aged 1 to 60 days of postnatal development (162 animals), about 6 animals
in each group. Three animals groups were studied on 1, 3, 7, 11, 14, 21,
30, 45, 60 days after bith. I gr. - intact animals (norm); ІІ gr. - control,
animals which were injected intrauterine 0.9% NaCl solution; III -
experimental animals injected with staphylococcal toxoid liquid purified
adsorbed (10-14 units of binding in 1 ml, diluted 10 portions) by operation
intrauterinely on the 18th day of dated pregnancy. Histological sections 3-5
μm thick were stained by hematoxylin and eosin, histochemicaly by alcian
blue and azan staining. Immunohistochemical study was performed
according to the protocol recommended for a particular antibody of the
manufacturer. Used ki-67 (Ki-67), TTF-1 (8G7G3/1), Fox-1 (A-12)
monoclonal antibodies by Santa Cruz Biotechnology, Inc. A set of
morphometric studies was performed by microscope Carl Zeiss Primo Star
equipped with the Axiocam digital microphoto attachment with using
program complex Zeiss Zen 2011. The results were considered reliable at
p≤0,05. For processing of statistical material was used the standard
software package Microsoft Office Excel and Statistica 10.0. |
URI: | http://dspace.zsmu.edu.ua/handle/123456789/22699 |
Располагается в коллекциях: | Наукові праці. (Гістологія, цитологія)
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