Immune Phenotypes of Endothelial-Derived Microparticles in Dysmetabolic

Abstract

Type two diabetes mellitus remains a leading contributor to cardiovascular mortality worldwide. This study was conducted to investigate the pattern of circulating endothelial-derived microparticles in diabetes patients in comparison with metabolic syndrome subjects. Methods: The study retrospectively evolved 101 patients (54 subjects with type two diabetes mellitus and 47 patients with metabolic syndrome) and 35 healthy volunteers. All the patients have given written informed consent for participation in the study. Biomarkers were measured at baseline of the study. Results: There is a significant difference between healthy subjects and patients regarding CD31+/annexin V+ to CD62E+ ratio of endothelial-derived microparticle, which reflects impaired phenotype of microparticles. Therefore, CD31+/annexin V+ to CD62E+ ratio was found to be higher in the type two diabetes mellitus patients compared to metabolic syndrome patients. Using multivariate linear regression analyses, independent impact of type two diabetes mellitus (r=0.40, P=0.003), OPG (r=0.37, P=0.001), hs-CRP (r=0.347, P=0.001), and adiponectin (r=0.33, P=0.001) on increased CD31+/annexin V+ to CD62E+ ratio of endothelial-derived microparticles was determined. Using C-statistics we found that inflammatory biomarkers (hs-C reactive protein, osteoprotegerin and adiponectin) added to the based model (type two diabetes mellitus) improved the relative integrated discrimination indices by 12.6% for increased CD31+/annexin V+ to CD62E+ ratio. In conclusion, we found that patients with type two diabetes mellitus and metabolic syndrome may distinguish predominantly appeared phenotypes of circulating endothelial-derived microparticles associated with pro-inflammatory cytokine over production. Elevated CD31+/annexin V+ to CD62E+ ratio is indicator of impaired immune phenotype of endothelial-derived microparticles, which allows determining pattern of microparticles in dysmetabolic disorder patients.